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Biomiga offers a variety of services including plasmid DNA purification, genomic DNA purificatiuon, mutation detection, mutagnesis, and gene synthesis /cloning for agricultural and medical samples. Plasmid DNA isolation • From small-scale ( < 1 mg) to large-scale ( 1 gram)
• Suitable for transfection and animal studies. • Free of guanidine salt, and ion exchange resin residues. • Low endotoxin level (< 0.2 EU per ¦Ìg DNA). • Free of Cesium Chloride and Ethidium Bromide. • Free of proteins, RNA and chromosomal DNA. Phage DNA isolation • Single & Double stranded M13 DNA Isolation
• Lambda phage growth and DNA purification • Custom bacteriophage prep • P1 Bacteriophage DNA Purification Subcloning and Degenerate-based gene cloning for cDNA and large duplication/deletion of gDNA • Cloning of unknown gene
• Cloning of gene of known sequences • Gene duplication and deletion Genomic DNA isolation •Greater than 40 kb in size after purification
•Purify from microbes, plants, mammalian tissues, and cultured cell lines. •Suitable for PCR, Southern blot, library constructions, SNP and other applications. Mutation detection by proofreading genotyping technology •We have developed a serial of SNP assay technologies that take advantage of high fidelity of exo+ polymerase with proofreading activity.
•As shown in the suggested model, mismatched amplicon is not extended and only perfectly matched amplicon is amplified, a so called on/off switch.
Mutagenesis •Point mutation on double stranded or single stranded DNA. •Deletion or duplication. Please contact us for details! |
