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Adenovirus Purification Maxi Kit 10 Preps V1260-02

v1260-02_640841505786_pto01
Regeneration Buffer, 2x Elution Buffer, 10x Wash Buffer, 50 mL Collection Tube, Desalting Tube, Press-On Cap, Maxi Columns {tab Description} Regeneration Buffer, 100 x Nuclease, Reaction Buffer,Nuclease, Binding Buffer, 15 mL Collection Tube, Desalting Tube, Press-On Cap, ...Read more
$1,030.00 each

Product Code: V1260-02


  • Product Details

Regeneration Buffer, 2x Elution Buffer, 10x Wash Buffer, 50 mL Collection Tube, Desalting Tube, Press-On Cap, Maxi Columns

Description

Regeneration Buffer, 100 x Nuclease, Reaction Buffer,Nuclease, Binding Buffer, 15 mL Collection Tube, Desalting Tube, Press-On Cap, Mini Columns

 

Introduction

The ViraTrapTM Adenovirus maxi purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x1012 viral particles can be purified from cell culture media of 6 to 8 T75 flasks.

 

Traditionally the recombinant lentivirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.

 

Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.

 

Storage and Stability

All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).

 

Before Starting

Familiar with each step by reading this menu and prepare all materials for the procedure.