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Description
Buffer GC, DNA Wash Buffer, Gel Elution Buffer, DNA Columns，Poly-Gel Filter Units, User Manual
The EZgeneTM Poly-Gel DNA Extraction Kit is designed for rapid and consistent recovery of ssDNA or dsDNA from acrylamide gels. Following PAGE, oligonucleotides are first visualized by UV shadowing or ethidium bromide staining and then cut out, soaked and eluted from using gel elution buffer. A specially formulated binding buffer is then added to the eluate to allow the binding of the DNA to the silica matrix. While proteins, salts, buffers, and other impurities are removed by wash buffer, the DNA is eluted sterile deionized water or low salt buffer. In addition, the spin column format allows parallel processing of multiple samples. The purified DNA is suitable for PCR, ligation reactions, hybridization techniques, or other procedures.
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