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1st Strand cDNA Synthesis System 5 preps FS01-00

{tab Description} Oligo(dT)20 (50 μM),Random hexamers (50 ng/μl),10? RT buffer*,25 mM MgCl2,0.1 M DTT,10 mM dNTP mix,SuperScript. III RT (200 U/μl),RNaseOUT. (40 U/μl),E. coli RNase H (2 U/μl),DEPC-treated water,Total HeLa RNA (10 ng/μl),Sense Control Primer (10 μM),A ...Read more
$40.00 each

Product Code: FS01-00


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Description

Oligo(dT)20 (50 μM),Random hexamers (50 ng/μl),10? RT buffer*,25 mM MgCl2,0.1 M DTT,10 mM dNTP mix,SuperScript. III RT (200 U/μl),RNaseOUT. (40 U/μl),E. coli RNase H (2 U/μl),DEPC-treated water,Total HeLa RNA (10 ng/μl),Sense Control Primer (10 μM),Antisense Control Primer (10 μM)

The First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg to 5 μg of total RNA. Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because Synthesis System for RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer. In the second step, PCR is performed in a separate