Search Store

Search

Product Categories

Total RNA Purification

No subcategories

In vivo study

No subcategories

Transfection Reagents

No subcategories

DNA Markers

No subcategories

Microbiology

No subcategories

Biomiga Vs. Competitor

No subcategories

Ni-NTA Agarose Resin 200 mL NI1211-01

{tab Description} 200 mL Ni-NTA Agarose Resin The IMAC medium BMG-Gel Ni consists of 90 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions. BMG-Gel Ni has low Ni2+ leakage, high protein-binding c ...Read more
$1,900.00 each

Product Code: NI1211-01


  • Product Details

Description

200 mL Ni-NTA Agarose Resin

The IMAC medium BMG-Gel Ni consists of 90 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions. BMG-Gel Ni has low Ni2+ leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. The medium is also suitable for gravity flow column purification and multiwell plate screening.

 

Medium characteristics:

Matrix

Highly cross-linked spherical agarose, 6%

Average particle size

90 μm

Metal ion capacity

~40 μmol Ni 2+/ml wet gel

Dynamic binding capacity a

>30 mg(Myoglobin)/ml wet gel

Recommended flow rate

150-300 cm/h

Max. operating pressure

0.3 MPa

Chemical stability b

0.01 M HCl、0.1 M NaOH(37 ℃, 7days);1 M NaOH、70%

acetic acid(12 h);30% 2-propanol(30 min);2% SDS(1 h)

pH stability b

2-14(Short term,2 h);3-12(Long            term,7days)

Storage

20% ethanol,4-30 ℃